VIABLE CRYOPRESERVED UMBILICAL TISSUE (VCUT) INHIBITS BACTERIAL GROWTH IN A SUBCUTANEOUS RAT INFECTION MODEL
Sandeep Dhall1, Turhan Coksaygan2, Tyler M. Hoffman1, Anne Lerch1, Jin-Qiang Kuang1, Malathi Sathyamoorthy1, Alla Danikovitch1
1Osiris Therapeutics Inc., Columbia, MD, USA, 2University of Maryland, Baltimore, Baltimore, MD, USA
Background: Surgical site infection (SSI) and adhesions are the most common complications contributing to substantial annual morbidity, costs, and deaths. SSI is the number one reason for hospital re-admission after surgery. Efficacy of currently available adhesion barriers remains poor, and their use is associated with increased rate of SSI. In Utero placental tissue is a barrier that protects developing fetus including protection from infection, and therefore, placental tissue might be an ideal biological material that prevents both adhesions and SSIs. Recently, a novel viable cryopreserved umbilical tissue (vCUT) adhesion barrier/wrap/cover for surgical procedures has been developed. vCUT retains all components found in their native state. Methods: In this study we tested antimicrobial activity of vCUT in a subcutaneous rat infection model. Bilateral 3cm dorsal incisions/animal (7animals/group), and a subcutaneous pocket was created at each incision site. vCUT or collagen dressing (control) was placed in each pocket. Bacterial inoculum of Escherichia coli(Gr-) or Staphylococcus aureus(Gr+) were added to each pocket before skin closure. 28days post-surgery each surgical site was visually evaluated, and tissue explants collected for microbiological and histological analysis. Explants were submerged in 1ml 0.9% sterile saline and vortexed for 1 minute to elute adherent bacteria from the tissue. Serial 10-fold dilutions were plated on blood agar and incubated at 37oC for 24hours before counting colony forming units (CFU). Results: Control animals (collagen dressing) developed subcutaneous abscesses at each surgical site (100%, 14/14). However, in the vCUT group the abscess was developed only at 3 sites (21%, 3/14). Degraded vCUT graft was still present at the site after 28 days as compared to the completely dissolved collagen dressing. Conclusion: vCUT inhibited bacterial growth and reduced incidence of abscess formation in an in vivo subcutaneous rat infection model. Data suggest that prevention of SSI might be one of the benefits provided by vCUT.
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