Myostatin-regulated Muscle Regeneration In Compressed Rat Skin
Takeo Minematsu, Sanai Tsunokuni, Ayano Nakai, Tamae Urai, Toshihiro Tsukatani, Hiromi Sanada.
The University of Tokyo, Tokyo, Japan.
[BACKGROUND] Skeletal muscle underlying skin is a vulnerable tissue with high regenerative activity. Its degeneration and regeneration might contribute to the development and healing of pressure ulcers. Myostatin (MSTN) is a major myokine that is constantly expressed in skeletal muscle to inhibit the overgrowth of muscle tissue. When muscle tissue is damaged, MSTN expression is inhibited, which promotes muscle regeneration. However, no studies have shown the contribution of MSTN for the development and healing of pressure ulcers. In this study, we investigated how pressure loading in rat skin influenced MSTN expression. [METHODS] The study protocols were approved by the Animal Research Committee of The University of Tokyo. We used 6-month-old male Sprague-Dawley rats. A metal plate was inserted subperitoneally and the overlying skin and muscle tissue were compressed using an indenter at 10 kg/3 cm2 for 6 hours under anesthesia. MSTN expression was examined using real-time RT-PCR, western blotting, and immunohistochemistry in the compressed and control skin tissue. [RESULTS] mRNA Mstn expression was detected in the control skin but not in the compressed skin. Western blotting and immunohistochemistry indicated the decreased expression of MSTN due to pressure loading. [CONCLUSION] These results suggest MSTN’s contribution in pressure ulcer development. Further studies to show the effects of the overexpression of MSTN in compressed skin are required.
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