Implantable Oxygen Platform For Continuous, Real-time Detection Of Vascular Perfusion And Ischemia
Mohamed M. Ibrahim, Ryan M. Schweller, Mahmoud M. Mohammed, David B. Powers, Bruce Klitzman.
Duke University Medical Center, Durham, NC, USA.
Lack of adequate perfusion can only be assessed post-operatively, after onset of tissue necrosis. We have developed new materials-based oxygen biosensor that can be implanted for deep and superficial measurements of tissue-oxygen-tension (TOT) in response to changes in perfusion.
Porphyrin-based sensors were incorporated into biocompatible hydrogels. To investigate TOT, sensors implanted intradermally and subcutaneously in rats. Sensor activity confirmed by modulating inspired oxygen levels between 12%-100%. Sensor modulation confirmed at 3,7,14 days post-implantation. Sensors similarly implanted in pigs to monitor TOT. To mimic ischemic-events, sensors directly injected at various-depths in pig-tongue which was then subject to tourniquet-ischemia.
Oxygen-sensors modulated appropriately to changes in oxygen-levels. In-vivo TOT could be modulated from 0-110mmHg by modulating inspired-oxygen between 12%-100%. Sensors could also be detected in non-perfused, ex-vivo human-skin via near-infrared-fluorescence using an imaging system(IVIS). Both fluorescence and lifetime-based measurements could be obtained after at least 1cm-deep-implantations. When implanted in pigs, sensors could be monitored at 5 anatomical sites simultaneously and permitted real-time monitoring of TOT during anesthesia and euthanasia. In swine-tongue, sensors immediately detected application
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