Decreased Lymphangiogenesis In The Skin Of Patients With Keloid
Guo-You ZHANG, Yi-Xuan ZHAO, Qing-Feng LI, Chao-Hua JIANG, Lian ZHU.
Shanghai Ninth People’s Hospital, Shanghai, China.
BACKGROUND: Vascular abnormalities are one of the primary pathological components of keloid. However, it has not been determined if there are also abnormalities in the formation of lymphatic vessels in keloid. The aim of this study is to evaluate lymphangiogenic activity in keloid skin. METHODS: Skin biopsies were collected from the involved skin of 11 patients with 11 and from the skin of 6 healthy volunteers. The numbers of D2-40, LYVE-1 and podoplanin -positive lymphatic vessels in skin specimens from healthy control subjects and patients with keloid were counted and compared. Quantitative real-time polymerase chain reaction (PCR) was performed to determine mRNA levels of the various splice variants of vascular endothelial growth factor, VEGF-A, VEGF-C and VEGF-D, their receptors VEGFR1, VEGFR2 and VEGFR3, TGF-β1, PROX1, LYVE1, podoplanin, bFGF. RESULTS: The number of lymphatic vessels in patients with keloid was significantly decreased compared with healthy control subjects (p<0.05). Mean relative transcript levels of FIGF (VEGF-D) and VEGFR3 in skin tissue from patients with keloid were significantly increased compared with healthy control subjects (p<0.05). Conclusions: A systemic increase of VEGF-D, as well as local overexpression of VEGFR3, may be the cause of disturbed lymphangiogenesis in keloid skin and play a role in the pathogenesis of keloid. We showed the possibility that regulation of VEGF-D ⁄ VEGFR3 signalling could lead to new treatment of skin ulcers in the keloid by controlling the formation of lymphatic vessels.
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