In Vitro Evidence Of Angiogenic Properties Of Fibrocytes By bFGF
Yoshikiyo Akasaka1, Chie Fujisawa1, Tetsuya Okaneya2, Miho Nakamichi2, Kiyoshi Onishi2, Yuri Akishima-Fukasawa3, Naoko Honma3, Tetuo Mikami3.
1Advanced Research Center, School Of Medicine, Toho University, Tokyo, Japan, 2Toho University Omori Medical Center, Tokyo, Japan, 3Toho University School Of Medicine, Tokyo, Japan.
Background: We have previously demonstrated the specific induction of CD34+/pro-collagen I+ fibrocytes for angiogenesis by basic fibroblast growth factor (bFGF) treatment in rat skin wounds. In this process, bFGF treatment markedly induced formation of capillary-like structures composed of CD34+/pro-collagen I+ fibrocytes in vivo. However, the in vitro evidence of fibrocyte induction for angiogenesis remains unclear. Methods and Results: To clarify the specific induction of this type of fibrocytes in vitro, we conducted collagen gel 3 dimensional culture incubated with bFGF-treated granulation tissue cells and found CD34+/procollagen+ double positive cells and capillary-like structures composed of the double positive cells in the gel. Significantly increased numbers of this type of fibrocyte were found in the collagen gel compared with the collagen gel cultured with non-treated granulation tissue cells after 7 days for treatment. We also found the capillary-like structures composed of CD34+/procollagen+ fibrocytes in the gel. Conclusion: This study demonstrated the capability of bFGF to induce angiogenic properties of fibrocytes in vitro, which provides new insight into mechanisms of angiogenesis resulting from fibrocyte expression during granulation tissue formation for healing.
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