Blood mRNA Integrity Isa Direct And Simple Reporter Of Radiation Exposure
Abdulnaser Alkhalil1, John I. Clifford2, Robert Ball1, Anna Day3, Stacy Ann Miller4, Ross Campbell5, Raina Kumar6, Rasha Hammamieh7, Lauren T. Moffatt8, Jeffrey W. Shupp9.
1Medstar Health Research Institute, Washington, DC, USA, 2Integrative Systems Biology, US Army Center for Environmental Health Research,, Fort Detrick, MD, USA, 3Firefighters’ Burn and Surgical Research Laboratory, The Oak Ridge Institute for Science and Education, Fort Detrick, MD, USA, 45Integrative Systems Biology, The Oak Ridge Institute for Science and Education, Fort Detrick, MD, USA, 56Advanced Biomedical Computational Science, Frederick National Lab for Cancer Research/Advanced Biomedical Computational, Fredrick, MD, USA, 6Advanced Biomedical Computational Science, Frederick National Lab for Cancer Research/Advanced Biomedical Computational, Fredrick, MD, USA, 7Integrative Systems Biology, US Army Center for Environmental Health Research, Fort Detrick, MD, USA, 8Firefighters’ Burn and Surgical Research Laboratory, MedStar Health Research Institute,, Washington, DC, USA, 93The Burn Center, Department of Surgery, MedStar Washington Hospital Center, Washington, DC, USA.
Background: Mass casualty caused by radiation requires fast assessment of patients’ health and sorting for optimal use of resources. Identifying level of exposure and hence the most suitable care is very challenging. We evaluated the impact of different radiation doses on mice using mRNA integrity in different specimens in search for changes marking outcome. Methods: Groups of mice (6-3 mice/group) received 0.5, 1, 5, 10, 20 Greys of radiation with or without a 20% burn injury. Animals were euthanized at 2hours, 12 hours, 1day, 2days, 3 days, 6days, 7 days, or 14 days post exposure. mRNA was isolated from blood and other tissue biopsies. The quality of mRNA was evaluated using bioanalyzer and RIN value were tallied. Results: Analysis of the integrity of mRNA showed that blood samples registered the best radiation dose-dependent damage. The damage either progressed by time and was not salvageable and associated with high mortality rates, or recovered after durations varied with the intensity of exposure. Combination with burns increased mortality significantly. Conclusions: These results provide a simple and accurate technique to guide health providers for best patients care administration under strenuous conditions of radiation mass causality.
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