Insights Into The Systemic Host Response To Deep Partial- And Full-thickness Burn Wounds Following P. Aeruginosa Infection In A Rat Burn Model
Alan Weaver, Jr., Kenneth Brandenburg, Liwu Qian, Shaina Van Stryk, Eliza Sebastian, Johnathan Abercrombie, Uzziel Pineda, Kai Leung.
United States Army Institute of Surgical Research, San Antonio, TX, USA.
Background: Bacterial infection accounts for 75% of fatalities in patients with a burn greater than 40% of their total body surface area (TBSA). This study investigated the systemic host response to Pseudomonas aeruginosa biofilm infection within deep partial-thickness (DPTB) and full-thickness burn (FTB) wounds using a scald rat burn model. Methods: DPTB or FTB wounds (~10% TBSA) were created in anesthetized male Sprague-Dawley rats using a modified Walker-Mason scald model (n=120). Immediately post-burn, the wound was inoculated with a clinical strain (strain 1244) of P. aeruginosa at 1x103 or 1x104 cells/wound). At 1, 3, 7, and 11 days post-burning, animals were euthanized and blood was collected for complete blood count, serum, and plasma analysis. Results: P. aeruginosa developed robust infections within both burn scenarios, having ~1x108 CFU/g of burn tissue within 7 days. Burns alone showed increases in neutrophils, monocytes, and basophils overtime, which were further elevated by the infection (particularly in FTB-104). These results were supported by cytokine profiling and may have also been influenced by both danger and pathogenic associated molecular patterns. Conclusions: A dynamically complex response was seen in both DPTB and FTB wounds with and without P. aeruginosa infection. These studies may provide insights to improve treatment methods for burn care. Disclaimer: The opinions or assertions contained herein are the private views of the author and not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
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