Effect Of Panax Ginseng Extract On The Activity Of Diabetic Fibroblasts In Vitro
Seung-Kyu Han1, Sik Namgoong1, Ji-Won Son2, Jae-youn Kim2.
1Korea University College of Medicine, Seoul, Korea, Republic of, 2Korea University Guro Hospital, Seoul, Korea, Republic of.
Background: Current studies on Panax ginseng have demonstrated that it has various medicinal properties including angiogenesis, immuno-stimulation, anti-microbial functions, and anti-inflammatory actions, which can be helpful in chronic wound healing. However, a direct role for P. ginseng in chronic wound healing has not been demonstrated. The present study was designed to evaluate the effects of P. ginseng extract on diabetic fibroblasts in vitro. Methods: For the experimental group, human diabetic fibroblasts were cultured in the presence of Ginsenoside Rb1 (G-Rb1), the active component in P. ginseng (10 ng/mL). No-G-Rb1-treated diabetic fibroblasts were used as controls. Cell proliferation, collagen synthesis, growth factor (basic fibroblast growth factor, bFGF; vascular endothelial growth factor, VEGF; transforming growth factor-ß1, TGF-ß1) production, matrix metalloproteinase 1 (MMP-1) synthesis, and tissue inhibitor of metalloproteinases 1 (TIMP-1) synthesis were compared using enzyme-linked immunosorbent assay (ELISA) kits and immunofluorescence staining. Results: In the ELISA, the G-Rb1-treated group was superior to the control group in cell proliferation, collagen synthesis, VEGF level, TGF-ß1 level, and TIMP-1 production by 48%, 20%, 47%, 14%, and 85%, respectively (p < 0.05). In the immunofluorescence staining, the G-Rb1-treated group was also superior to the control group in cell proliferation, collagen synthesis, VEGF level, TGF-ß1 level, and TIMP-1 production by 34%, 46%, 80%, 72%, and 164%, respectively (p < 0.05). However, both ELISA and immunofluorescence staining revealed that differences in bFGF and MMP-1 levels between the two groups were not statistically significant. Conclusion: P. ginseng treatment may stimulate the wound healing activity of diabetic fibroblasts in vitro.
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