Wound Healing Society

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Developing A Knitted, Antibacterial Wound Dressing Contact Layer For Infection Management
Elizabeth Gianino, Damea Pham, Jordon Gilmore.
Clemson University, Clemson, SC, USA.

The purpose of this study is to develop the skin-contact layer of a tri-layered wound dressing with optimal material and geometric properties needed to deliver gentamicin sulfate (GS) for the effective control of pathogenic Pseudomonas. Poly-l-lactide (PLA), poly-ε-caprolactone (PCL), and copolymer (PLC) were characterized using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Fiber monofilaments were fabricated using a lab-scale continuous twin screw melt-extruder and spinneret system (Alex James and Associates, Inc.; Greenville, SC). Tensile strength testing was performed to rate fiber properties conducive to knitting. A 34-multifilament PLA yarn was extruded with Stantex® spin finish and the elastic modulus significantly differed (p<0.08) from the monofilament. PLA yarn exhibited resistance to breakage needed for knitting and was chosen as the contact layer material. Contact layers were soaked in solutions between 1μg/ml and 10,000μg/ml of GS. Drug elution was determined by a modified dialysis membrane method, where contact layers were submerged in PBS with the release measured via fluorescent spectrometry using o-Pthaldialdehyde. Contact layers were placed into active cultures of P. putida. Antibacterial efficacy was determined by measuring the optical density over five days. Each soaking condition had a burst release after 5 hours. The elution profile for all conditions released antibiotic over the minimum inhibitory concentration (MIC) against P. putida. The antibacterial experiments showed that GS exhibited both cidal and static antibacterial effects by killing bacteria and further inhibiting growth, respectively. The control contact layer showed minor bacterial inhibition of growth indicating that spin finish might have antibacterial properties. This study explores the ideal biomaterial’s resistance to breakage needed for knitting. The elution profile exhibited a burst release over short time intervals, where antibiotic effectiveness remains highest.

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