Electric Field Based Wound Dressing Is Effective In Managing Candida Albicans Yeast Infection
Suman Santra, Dolly K. Khona, Chandan K. Sen.
Indiana University School of Medicine, Indianapolis, IN, USA.
Background: Candida albicans (CA) are common opportunistic pathogen that causes skin infections, mucosal infection or systematic infections in immunocompromised or neutropenic patients. Recent report show that electric field based therapies are effective to manage biofilm infection. In this work we tested the effect of similar electric field on the growth of CA. CA used for this study was isolated from human mouth swab and is known to be resistant to most fungicides (ATCC 64124). Method: A United States Food and Drug Administration-cleared wireless electroceutical dressing (WED) was tested. WED relies on electrochemistry enabled by Ag/Zn printed on polyster fabric to generate weak electric field. The same fabric excluding the printed metal served as control. The final stock of yeast used for experiments was 8 x 108 cells/ml. CA was treated with WED or the above-mentioned control followed by culture in Yeast Extract Peptone Dextrose (YPD) agar employing pour plate technique. Log reduction was calculated using the formula Log Reduction = Log10(U0) - Log10(Tt ).Result: Under control conditions, following 24h of culture 70 x 106 colonies were observed (n=3, two independent experiments). However, exposure to WED resulted in complete abrogation of all colonies in each of the six plates (n=3, two independent experiments). Conclusion: This work provides first evidence that WED is effective in managing pathogenic yeast infection. Clinical studies testing WED in a clinical setting for the management of yeast infection are warranted.
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