Longitudinal Transcriptome Analyses Of Burn Wounds In Red Duroc Vs. Yorkshire Pig
Jesse Nguyen1, Andrea Fourcaudot2, Sai Lakshmi Rajasekh Karna1, Fatemah Sanjar3, Fatemah Sanjar3, Liwu Qian1, Ping Chen1, David Todd Silliman1, Stardaous Gibbons1, Kai P. Leung1.
1Brooke Army Medical Center, Fort Sam Houston, TX, USA, 2Andrea Fourcaudot, Fort Sam Houston, TX, USA, 3Brooke Army Medical Center, San Antonio, TX, USA.
Longitudinal transcriptome analyses of burn wounds in red Duroc vs. Yorkshire pig
Background:Hypertrophic scar (HTS) is characterized by firm, raised lesions, and is one of the adverse outcomes of deep-partial thickness (DPT) dermal burn injuries. HTS results in contractures causing negative-functional and aesthetic consequences while hindering full recovery and quality of life. HTS is associated with unique gene expression profiles, however, the dynamics of global gene expression during the course of hypertrophic scarring is not well characterized.
Method:RNA sequencing was used to determine the total transcriptome of superficial, deep-partial burn wounds and unburned tissue isolated on days 0, 3, 7, 15, 30 and 60 post-burn from red Duroc (prone to scarring) and Yorkshire pigs (less prone to scarring). Differentially-expressed genes (DEG) between burn types and time-points were analyzed to determine unique gene expression profiles. K-mean analyses were performed to cluster longitudinal gene expression profiles based on temporal trends.
Results:Shared DEGs between superficial and DPT burn wounds increased from 62 to 616 genes as the wound progressed from D0 to D3 and stabilized on D7 with 601 shared DEGS. K-mean clustering analyses of the burn types between Yorkshires and Durocs categorized 3,903 genes based on expression profiles and temporal trends.
Conclusion:We have detected DEGs between superficial and deep-partial genes temporally in Yorkshires and Durocs. These genes may serve as biomarkers for future therapeutic studies.
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