First Insight Into The Wound Archaeome Archaea Communities In Epidemiologically Diverse Collection Of Human Chronic Wounds
Fatemeh Sanjar1, Marcus L. Gitterle2, Kai P. Leung1.
1Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, San Antonio, TX, USA, 2Christus Santa Rosa Hospital, New Braunfels, TX, USA.
Background: To date, there has been no studies aimed at identifying the archaea residents of the human chronic wounds and contributions of archaeal residents of the human microbiome to the microbiome community function and the human health is largely unknown. As essential inhabitants of the human gut microbiome, the gut archaeome harbors keystone species attributed to critical metabolic processes for the microbiome. Methods: Using high-resolution whole-metagenomic sequencing of a diverse collection of human chronic wounds (venous leg ulcer, diabetic foot ulcer, pressure ulcer, and post-surgery dehisced wounds) and based on the available archaea sequence databases, 40% of the 45 human chronic wounds detected the presence of archaeal sequences, at varying abundance. The repertoire of the chronic wound archaeome exhibited similarities to the archaeal community profile associated with the more studied human gut microbiome, in particular during disease (e.g., Irritable bowel syndrome). Majority of the chronic wound archaeome belonged to Euryarchaeota followed by Crenarchaeota family. Dominant members were classified as methanogenic, acidophilic, and halophilic archaea. Conclusion: Our findings provide initial bases for further investigation of the human wound archaeome and discovery of potential functional roles (e.g., metabolic) of archaea in human wound healing processes and state of the chronic wound microbiome. DOD disclaimer. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.
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