Wound Healing Society

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Do Human Beta Defensins (hbd) Play A Role In Keloid Pathogenesis
Khurshid Alam.
Queen Victoria Hospital, New Malden, United Kingdom.

Abstract Keloids are abnormal scar giving a disfiguring appearance and extending beyond the original wound. Keloids have a multi-factorial pathogenesis where a number of factors interplay to form these lesions in individuals with higher genetic predisposition. The multi-factorial pathogenesis can be summarized as either an increased exposure to and /or expression of inflammatory mediators. Human Beta Defensins (HBD) are a group of innate immune peptides which although originally considered to be primarily anti-microbial in function have been identified to have diverse functions. In this study we investigate the expression of these immune peptides in Keloids. Methods.Keloid sample from different parts in 10 patients were analyzed for expression of HBD-1,2,3 at Protein level and mRNA levels. The expression at mRNA level was assessed using real tome duplex rt-PCR and protein expression was done using immunohistochemistry. The Expression was analyzed in relation to normal skin sample as control. Results None of the Keloid sample expressed HBD2 at mRNA and protein levels. While mean expressions of HBD-1 and 3 both at mRNA and protein levels were 3 and 5 times higher than the normal skin samples around the Keratinocytes. Conclusion-For the first time the study demonstrates the expression profile of HBD in Keloid. The absence of HBD-2 expression at both mRNA and protein levels suggest a possible role of these peptide in Keloid pathogenesis. The study also alludes to the possible diverse role of HBD peptides apart from being antimicrobial.


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