The Effect OfHuman Induced Pluripotent Stem Cell-derivedEcfcsFor Angiogenesis & Granulation Tissue Formation
EunSoo Park, SangHun Kim, JeoungHyun Nam, YuGil Park.
Soonchunhyang University Bucheon Hospital, Bucheon/Gyeonggi-do, Korea, Republic of.
BACKGROUND: Human induced pluripotent stem cells(hiPSC) complement the disadvantages of conventional embryonic stem cells and adult stem cells, and have the advantages of simplicity of cells production technology and pluripotency. Recently, some studies about hiPSC has been done in the field of cell therapy. METHODS: In this study, we examined the effect of cells similar to CB-ECFCs differentiated from induced pluripotent stem cells about angiogenesis and granulation tissue formation in proliferative phase of wound healing. For cell transfer, GelMA-co-PSS cryogel was used, which has better bioactivity than conventional hydrogels and has excellent mechanical properties and swelling capacity. Two full - thickness skin defects of 0.8 cm in diameter were injected into each of 12 experimental mouses. Wound splinting model was used to inhibit wound contraction. In each of the experimental animals, 5x105 cells were applied with GelMA-co-PSS cryogel in one of the two wounds, and the other wound was applied only culture medium with the cryogel. RESULTS: Wound reduction rates had showed significant increase in 3 days when the experimental group compared to the control group (p<0.10) and the histological score was also significantly increased (p <0.05). Histologic examination and immunohistochemical staining showed that angiogenesis and granulation formation were increased in the experimental group. CONCLUSIONS: In conclusion, cord-blood endothelial colony-forming cell-like cells differentiated from human induced pluripotent stem cells were effective to promote angiogenesis and granulation tissue formation in mouse wound healing model.
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