Action Of Monocyte Chemoattractant Protein 1 On Adipose-derived Stem Cells
Anesh Prasai, Amina El Ayadi, Jayson W. Jay, Perenlei Enkhbaatar, Osamu Fujiwara, David N. Herndon, Celeste C. Finnerty.
University of Texas Medical Branch, Galveston, TX, USA.
Adipose-derived stem cells (ASCs) have been studied for their ability to augment wound healing. To understand their role, we applied ASCs topically to the burn wounds in an ovine model of burn. Application of ASCs significantly increased expression of the vascular endothelial growth factor (VEGF) (p<0.05) and collagen-1 (p<0.01) proteins. Expression of monocyte chemo-attractant protein-1 (MCP-1) mRNA transcripts was significantly higher with ASC treatment compared to untreated (p<0.05). MCP-1 has been implicated in wound healing as well, therefore we set out to elucidate the role of MCP-1 in processes underlying wound healing, including angiogenesis and cell migration. ASCs (n=3) express significantly greater basal levels of MCP-1 compared to bone marrow-derived stem cells or fibroblasts (each p<0.05). To determine the effect of MCP-1 on angiogenesis, human umbilical vein endothelial cells (HUVECs) were treated with 100 ng/mL MCP-1 or 2% ASC-conditioned-media (CM). Expression of MCP-1, VEGF-165, and HIF-1α mRNA transcripts increased with both treatments (p<0.05), confirming that MCP-1 works in an autocrine fashion. Similarly, fibroblast migration increased significantly with either 2% ASC-CM or MCP-1 treatment (p<0.001). In conclusion, we found that ASCs constitutively produce abundant levels of MCP-1, which may be responsible for their therapeutic properties, and that this increase in MCP-1 expression may underlie increased angiogenesis and cell migration.
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