The Polarization Of Th2 Response Improves The Healing Of Deep Partial-thickness Burns
Edna A. Mandujano, YI1, Francisco González1, Rosa M. Salgado1, Gerardo Arrellín2, Edgar Krötzsch1.
1Instituto Nacional de Rehabilitación, Mexico, Mexico, 2Universidad Autónoma de Morelos, Mexico, Mexico.
BACKGROUND: An imbalance in the local and systemic inflammatory response guides to an impaired healing process of wounds. In this sense, it is suggested that severe burn patients show an enhanced and chronic type 2 T-helper cell (Th2) response, while Th1 response is early suppressed. Therefore, it is imperative to elucidate the effect of this Th2 inflammatory response during the burn healing.
METHODS: We performed a model in which 8 weeks-Balb/c mice were irritated and challenged with toluene diisocyanate (TDI) to polarize the Th2 response. To evaluate the effect of Th2 polarization on the healing of burns, we generated a deep partial-thickness burn on TDI mice, and then we histologically characterized all the stages of the healing process and evaluated cytokines Th1 and Th2 levels by qRT-PCR and immunohistochemistry.
RESULTS: In order to validate our model, we measured mRNA levels of the Th2-type cytokines (IL-13, IL-10 and IL-4) as well as the Th1-type cytokines (IFN-ɣ, TNF-α and IL-2) in skin tissue and inguinal nodes. We found that after the irritation and challenge, mice exhibited increased levels of Th2-type cytokines in both, skin tissue and inguinal nodes, indicating that the induction of a polarized Th2 response was effective. Interestingly, Th2 polarized mice exhibited an improvement in the reepithelization stage compared with the control group. At the molecular level, treated mice showed increased levels of Th2-type cytokines, from one day post-burn (1 dpb) to 28 dpb, while this anti-inflammatory response decreased over time in control mice. Furthermore, the presence of IL-13 in the stroma was clearly high in TDI mice at early stages of the wound healing.
CONCLUSIONS: Our results suggest that an exacerbated anti-inflammatory response, over the normal condition, promotes the healing of burns.
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