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A NOVEL MURINE DISTRACTION DEVICE INVESTIGATING LONG BONE REGENERATION
Harsh N. Shah, Ankit Salhotra, Michael T. Lopez, Derrick C. Wan, Michael T. Longaker.
Stanford University, Stanford, CA, USA.

BACKGROUND: The application of distraction osteogenesis (DO) has revolutionized the treatment of many congenital and acquired defects. Here, we describe the development of a novel mouse distraction model for the tibia.
METHODS: Tibial distraction devices were designed using computer-aided design software and 3D-printing. Once 0.6mm hole was drilled on either side of the tibial crest. An osteotomy was performed at the tibial crest using a diamond disc saw. Distraction plates were secured with 0.65mm screws. Animals were divided into four groups: sham (without osteotomy), fracture (osteotomy without distraction), acutely lengthened, and gradually distracted. The gradual distraction protocol consisted of a five day latency period, followed by 10 days of distraction at a rate of 0.15mm every 12 hours, and 28 days of bone consolidation. For our acute lengthening protocol, a 3.0mm lengthening was performed following a five day latency period.
RESULTS: Micro-computed tomography images of the sham group presented native, unperturbed bone, while the acute lengthening group showed the absence of bone regeneration at the site of the osteotomy. Bone regeneration occurred in the fracture group and the gradual distraction group. Upon quantitative analysis, the bone volume per tissue volume (BV/TV) (***P<0.001) and callus volume (****P<0.0001) were significantly higher in the distraction group compared to the sham group.
CONCLUSIONS: We have developed a new model for long bone distraction osteogenesis in the mouse to determine the underlying bone regeneration mechanisms.


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