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Anthocyanins From Black Soybean Seed Coat Prevent Radiation-Induced Skin Fibrosis By Downregulating Tgf-? And Smad3 Expression
Jaehoon Choi
Department of plastic and reconstructive surgery, Keimyung University School of Medicine, Daegu, Daegu, Korea (the Republic of)

Background: The aim of this study was to evaluate the protective effects of anthocyanins from the black soybean seed coat against radiation injury in dermal fibroblasts and mouse skin.
Methods: Dermal fibroblasts treated with 50 and 100 ?g/mL anthocyanins were irradiated with single doses of 20 Gy. Cell viability, intracellular reactive oxygen species (ROS) production, and mRNA expression were measured. A total of 60 mice were used for an in vivo study. A dose of 100 ?g/mL anthocyanins was administered daily for 5 days before or after radiation therapy. Following irradiation (45 Gy), mice were inspected for gross pathology twice per wk for 8 weeks. At 4 and 8 weeks post-irradiation, dorsal skin was harvested for histopathologic examination and protein isolation.
Results: In dermal fibroblasts, treatment with 50 and 100 ?g/mL anthocyanins significantly reduced radiation-induced apoptosis at 72 h and intracellular reactive oxygen species generation at 48 h. Furthermore, 100 ?g/mL anthocyanins markedly decreased Smad3 mRNA expression and increased Smad7 mRNA expression at 72 h post-irradiation. In mice, treatment with 100 ?g/mL anthocyanins resulted in a significant reduction in the level of skin injury, epidermal thickness, and collagen deposition after irradiation. Treatment with 100 ?g/mL anthocyanins significantly decreased the number of ?-SMA-, TGF-?-, and Smad3-positive cells after irradiation.
Conclusion: Our study demonstrated that black soybean anthocyanins inhibited radiation-induced fibrosis by downregulating TGF-? and Smad3 expression. Therefore, anthocyanins may be a safe and effective candidate for the prevention of radiation-induced skin fibrosis.


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